Tagging of avidin immobilized beads with biotinylated YAG:Ce3+ nanocrystal phosphor |
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| Authors: | Ryo Asakura Tetsuhiko Isobe Kiyoshi Kurokawa Hideki Aizawa Michio Ohkubo |
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| Institution: | (1) Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan;(2) Department of Anatomy, Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu-shi, Shiga 520-2192, Japan;(3) Furukawa Electric Co., Ltd., 2-4-3 Okano, Nisi-ku, Yokohama 220-0073, Japan |
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| Abstract: | YAG:Ce3+ nanoparticles 9.5 ± 1.2 nm in diameter have been synthesized from aluminium isopropoxide and acetates of yttrium and cerium
in 1,4-butanediol (1,4-BD) by autoclave treatment at 300 °C for 2 h. After replacing 1,4-BD by ultrapure water, NH2 groups were introduced on the surface of YAG:Ce3+ nanoparticles by addition of 3-aminopropyltrimethoxysilane then biotinylation with sulfo-NHS-LC-biotin. We demonstrated that
avidin immobilized beads are tagged by biotinylated YAG:Ce3+ nanoparticles by the selective avidin-biotin interaction, furnishing a green fluorescent image on excitation with blue light.
This result indicates that YAG:Ce3+ nanoparticle phosphors have much potential in biological applications. |
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| Keywords: | YAG:Ce3+ Nanoparticle 3-aminopropyltrimethoxysilane Avidin-biotin interaction Fluorescent image Biological application |
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