Degradation of eucalypt waste components by <Emphasis Type="Italic">Lentinula edodes</Emphasis> strains detected by chemical and near-infrared spectroscopy methods |
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Authors: | M Brienzo E M Silva A M F Milagres |
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Institution: | (1) Dept. of Biotechnology, Escola de Engenharia de Lorena-USP, CP 116 CEP 12 602 810 Lorena-SP, Brazil |
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Abstract: | There are many changes, both qualitative and quantitative, in eucalypt waste during growth and fructification of Lentinula edodes. Wet chemical analysis and near-infrared (NIR) spectroscopy were used in conjunction with multivariate regression and principal
components analysis to monitor biodegradation of eucalyptus waste during growth of several L. edodes strains. Weight and component losses of eucalypt residue after biodegradation by L. edodes strains were compared for periods of 1 to 5 mo. Decrease in cellulose, hemicellulose, and lignin contents occurred, however
it was not concomitant. Measurement of lignin degradation by NIR and wet chemical analysis indicated its attack in the early
stages of biodegradation. Selective lignin degradation by L. edodes was observed up to 2 mo of biodegradation for strains DEBIQ and FEB-14. One group of degraded substrate was identified based
on the principal component analysis (PCA) of the data on their biodegradation time. Samples treated for 5 months by L. edodes strains (DEBIQ, UFV or FEB-14) differed from other, but no discrimination was observed among them. By the end of 5 mo, NIR
analyses showed decrease of about 18–47% cellulose, 35–47% polyose and 39–60% lignin. These data were used for comparison
with those obtained by wet chemical method for the degradation of the substrate by other five L. edodes strains cultivated at the same conditons. NIR calibration developed in this study was proven to be perfectly suitable as
an analytical method to predict the changes in lignocellulose composition during biodegradation. |
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Keywords: | Lentinula edodes biodegradation Near infrared lignin selectivity |
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