Tumor-Cell-Specific Targeting of Ibrutinib: Introducing Electrostatic Antibody-Inhibitor Conjugates (AiCs) |
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Authors: | Andreas Faust Nicole Bumer Alina Schlütermann Manuel Becht Lilo Greune Christiane Geyer Christian Rüter Renato Margeta Lisa Wittmann Petra Dersch Georg Lenz Wolfgang E Berdel Sebastian Bumer |
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Institution: | Andreas Faust,Nicole Bäumer,Alina Schlütermann,Manuel Becht,Lilo Greune,Christiane Geyer,Christian Rüter,Renato Margeta,Lisa Wittmann,Petra Dersch,Georg Lenz,Wolfgang E. Berdel,Sebastian Bäumer |
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Abstract: | Ibrutinib is an inhibitor of Bruton's tyrosine kinase that has been approved for the treatment of patients with chronic lymphocytic leukemia, mantle cell lymphoma and Waldenstrom's macroglobulinemia and is connected with toxicities. To minimize its toxicities, we linked ibrutinib to a cell-targeted, internalizing antibody. To this end, we synthesized a poly-anionic derivate, ibrutinib-Cy3.5, that retains full functionality. This anionic inhibitor is complexed by our anti-CD20-protamine targeting conjugate and free protamine, and thereby spontaneously assembles into an electrostatically stabilized vesicular nanocarrier. The complexation led to an accumulation of the drug driven by the CD20 antigen internalization to the intended cells and an amplification of its pharmacological effectivity. In vivo, we observed a significant enrichment of the drug in xenograft lymphoma tumors in immune-compromised mice and a significantly better response to lower doses compared to the original drug. |
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Keywords: | antibodies Bruton's kinase inhibitor drug delivery electrostatic nanocarriers protein engineering vesicles |
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