用于硫酸庆大霉素高效液相色谱分离-电化学测定的四电位波型的优化

提出并优化了用于庆大霉素测定的四电位检测波型，建立了庆大霉素的高效液相色谱分离-电化学测定新方法。在该新检测波型中，使用了负的清洗电位，该清洗电位具有清洗效果好，对金工作电极的氧化溶解损伤小，因而建立的分析方法具有满意的精密度。该分析方法已经被应用于两种实际样品的测定。

Optimizing the Quadruple-potential Waveform for the Determination of Gentamicin Sulfate by High Performance Liquid Chromatography with Pulsed Electrochemical Detection

In this paper, a quadruple-potential waveform was investigated and optimized for the determination of gentamicin by reversed phase ion-pair chromatography. Instead of a relatively high positive potential, a negative potential was adopted as a potential for the cleaning of gold working electrode. By this way, the formation of gold oxide resulting from the application of high positive potential during the analyte detection and electrode cleaning was greatly reduced, and therefore, the dissolution and recession of gold working electrode was also reduced. The good condition of gold working electrode achieved by this quadruple-potential waveform can help us to obtain a good reproducibility. In order to acquire signal-to-noise ratio as high as possible, several waveform parameters affecting the detection of gentamicin were carefully selected. The analytical method has been applied to the determination of two real gentamicin samples, and good results with low relative standard deviation not more than 4% were obtained.