Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates |
| |
Authors: | Heikkinen Jarkko J Kivimäki Liisa Määttä Juha A E Mäkelä Inka Hakalahti Leena Takkinen Kristiina Kulomaa Markku S Hytönen Vesa P Hormi Osmo E O |
| |
Affiliation: | Department of Chemistry, University of Oulu, Oulu, Finland. jarkko.j.heikkinen@oulu.fi |
| |
Abstract: | A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol-gel substrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3-aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide-N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol-gel modified PMMA film. Different concentrations of chimeric avidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimeric avidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |