Immunomodulatory Responses of Two Synthetic Peptides against Salmonella Typhimurium Infection |
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Authors: | Marco Antonio Ibarra-Valencia Gerardo Pvel Espino-Solis Blanca Elisa Estrada Gerardo Corzo |
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Institution: | 1.Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, A.P. 510-3, Cuernavaca 62250, Mexico;2.Laboratorio de Investigación Traslacional and Laboratorio Nacional de Citometría de Flujo-UACH, Universidad Autónoma de Chihuahua, Circuito Universitario, Campus II, Chihuahua 31109, Mexico; (G.P.E.-S.); (B.E.E.) |
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Abstract: | In vitro assays of phagocytic activity showed that the peptide Pin2G] stimulates phagocytosis in BMDM cells from 0.15 to 1.25 μg/mL, and in RAW 264.7 cells at 0.31 μg/mL. In the same way, the peptide FA1 induced phagocytosis in BMDM cells from 1.17 to 4.69 μg/mL and in RAW 264.7 cells at 150 μg/mL. Cytokine profiles of uninfected RAW 264.7 showed that Pin2G] increased liberation TNF (from 1.25 to 10 μg/mL) and MCP-1 (10 μg/mL), and FA1 also increased the release of TNF (from 18.75 to 75 μg/mL) but did not increase the liberation of MCP-1. In RAW 264.7 macrophages infected with Salmonella enterica serovar Typhimurium, the expression of TNF increases with Pin2G] (1.25–10 μg/mL) or FA1 (18.75–75 μg/mL). In these cells, FA1 also increases the expression of IL-12p70, IL-10 and IFN-γ when applied at concentrations of 37.5, 75 and 150 μg/mL, respectively. On the other hand, stimulation with 1.25 and 10 μg/mL of Pin2G] promotes the expression of MCP-1 and IL-12p70, respectively. Finally, peptides treatment did not resolve murine gastric infection, but improves their physical condition. Cytokine profiles showed that FA1 reduces IFN-γ and MCP-1 but increases IL-10, while Pin2G] reduces IFN-γ but increases the liberation of IL-6 and IL-12p70. This data suggests a promising activity of FA1 and Pin2G] as immunomodulators of gastric infections in S. Typhimurium. |
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Keywords: | antimicrobial peptides Salmonella enterica serovar Typhimurium cytokines inflammation scorpion peptides |
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