Study on selectivity of permeating chiral complexes in bilayer lipid membranes (BLMs) |
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| Institution: | 1. Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital-The Norwegian Radium Hospital, 0379 Oslo, Norway;2. Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, 0379 Oslo, Norway;3. Department of Molecular Biosciences, University of Oslo, 0316 Oslo, Norway;1. Adelaide Medical School and Freemasons Foundation Centre for Men''s Health, University of Adelaide, Adelaide, SA 5005, Australia;2. South Australian Health and Medical Research Institute, Adelaide, SA 5000, Australia;3. Department of Surgery and Cancer, Imperial College London, Imperial Centre for Translational and Experimental Medicine, London, UK;4. Laboratory of Lipid Metabolism and Cancer, KU Leuven Cancer Institute, 3000 Leuven, Belgium;5. Program in Experimental and Molecular Medicine, Geisel School of Medicine at Dartmouth, 1 Medical Center Drive, Lebanon, NH 037560, USA;6. Pathology and Laboratory Medicine, Weill Cornell Medical College, Cornell University, New York, NY 10065, USA;7. The Norris Cotton Cancer Center, Geisel School of Medicine at Dartmouth, 1 Medical Center Drive, Lebanon, NH 03756, USA |
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| Abstract: | Electrical properties such as membrane potential (Em) of planar bilayer lipid membranes (BLMs) are readily measured. Planar BLMs have been extensively used as models of biomembranes. In this paper we report BLMs formed in the solutions containing chiral complexes: d-KCo(EDTA)], l-KCo(EDTA)]; d-Co(C2O4)(en)2]I, and l-Co(C2O4)(en)2]I, whose Em values display great differences, implying strong chiral selectivity. The permeability ratios of different chiral complexes calculated from Em are the same as those obtained from human erythrocyte experiments. These results showed that chiral selectivity of cell uptake was mainly caused by the chirality of the membrane phospholipid itself. As a rapid and sensitive analytic tool, the BLM may be used to study permeating pathways and drug–membrane interactions. With further research, the BLM system may be developed into a useful method for drug screening. |
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