Fabrication of an integrated PDMS microchip incorporating an LED-induced fluorescence device |
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Authors: | Kyo?Miyaki Yanli?Guo Takuya?Shimosaka Tatsuro?Nakagama Hizuru?Nakajima Email author" target="_blank">Katsumi?UchiyamaEmail author |
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Institution: | (1) Department of Applied Chemistry, Graduate School of Engineering, Tokyo Metropolitan University, 1-1, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan |
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Abstract: | A microfluidic device with an integrated fluorescence detection system has been developed in order to miniaturize the entire analytical system. A blue or green light-emitting diode (LED) and an optical fiber were mounted in a polydimethylsiloxane-based microchip. The performance of this device was evaluated by microchip electrophoresis. When a green LED was used as the light source, the calibration curve of Sulforhodamine-101 was linear over the range 1–100 M. The detection limit was found to be 600 nM (240 amol) for a S/N ratio of 3. When using a blue LED, the calibration curve of Fluorescein was linear over the range 0.2–100 M. The detection limit was estimated to be 120 nM (50 amol) (S/N=3). The detection sensitivity per unit power was comparable to that of LIF. The RSD values for the migration time, peak height and peak area were 0.74, 7.18 and 9.45%, respectively. The integrated microfluidic device was successfully used to determine amino acid derivatives. |
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Keywords: | Integrated PDMS microchip Light-emitting diode Fluorescence Fabrication method Microchannel electrophoresis |
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