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| 氯化血红素作为过氧化物模拟酶与HRP的比较研究 | |
| 引用本文： | 郑宁,葛晨灏,郭忠先,李元宗,常文保,王宗睦,李铁津. 氯化血红素作为过氧化物模拟酶与HRP的比较研究[J]. 分析科学学报, 2001, 17(4): 265-269 |
| 作者姓名： | 郑宁 葛晨灏 郭忠先 李元宗 常文保 王宗睦 李铁津 |
| 作者单位： | 1. 北京大学化学与分子工程学院;吉林大学化学系 2. 北京大学化学与分子工程学院, 3. 吉林大学化学系, |
| 基金项目： | 教育部“跨世纪优秀人才培养计划”基金，中国博士后科学基金 |
| 摘 要： | 用荧光光谱和紫外－可见吸收光谱研究了氯化血红素（hemin)催化过氧化氢与对羧基苯丙酸反应的动力学和反应产物，表明hemin同时具有模拟过氧化物酶、过氧化氢酶的活性，在反应过程中hemin自身被氧化分解。hemin催化反应的产物除与天然的辣根过氧化物酶（HRP）催化的产物相同（λex/λem=310nm/406nm）外，还有激发峰在258nm和300nm，发射峰在360nm的荧光物质。通过对产物光谱特性的比较，研究了hemin和HRP的特异性差异。该研究结果对进一步筛选和研究高特异性过氧化物模拟酶具有重要的指导意义。 |
| 关 键 词： | 氯化血红素 特异性 模拟酶 催化荧光反应 辣根过氧化物酶 过氧化氢 对羟基苯丙酸 光谱 |
| 文章编号： | 1006-6144(2001)04-0265-04 |
| 修稿时间： | 2000-05-09 |
Comparative Studies on Hemin as a Mimic of Peroxidase with Horseradish Peroxidase |
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| Abstract: | The reaction kinetics and spectral characteristics of products of the hemin catalyzed oxidation of p hydroxyphenylpropionic acid with hydrogen peroxide were studied through fluorometry and spectrophotometry. Hemin was found to show peroxidase like, catalase like activities, and to be decomposed due to oxidation and dimerization. Besides the primary product with maximum emission at 406 nm when excited at 320 nm, the system has a fluorescent by product with an emission peak at 360 nm when excited at 258 or 300 nm. The difference in specificity of hemin and horseradish peroxidase was examined by comparing spectral characteristics of reaction product. The competitive relationship of the multiple roles of hemin in the system was discussed. The result is of importance in mimicry of peroxidase with high specificity. |
| Keywords: | Hemin Specificity Mimic of enzyme Catalytic fluorogenic reaction Horseradish peroxidase |
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