Gazi Üniversitesi, Fen-Edebiyat Fakültesi, Kimya Bölümü, 06500 Ankara, Turkey
Abstract:
The polarographic reduction of lead in the presence of selenite gives rise to an additional peak corresponding to the reduction of lead (Pb) on adsorbed selenium (Se) on mercury at −0.33 V. The selenium and lead content can be determined using this peak by the addition of a known amount of one of these ions first and then the second ion. The linear domain range of lead is 5.0×10−7–2.0×10−5 M and for selenium 5.0×10−7–1.0×10−5 M. Using this method 4.90×10−7 M Se(IV) and 1.47×10−6 M Pb(II) in a synthetic sample could be determined with a relative error of +2.0% and 1.8%, respectively (n=4). A recovery test after acid digestion for a synthetic sample was 97% for selenium and 96.5% for lead. The method was applied to 1 ml of digested blood, and 328±23 μg l−1 Se(IV) and 850±62 μg l−1 Pb(II) could be determined with a 90% (n=5) confidence interval.