Continuous production of butanol by clostridium acetobutylicum immobilized in a fibrous bed bioreactor |
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Authors: | Wei-Cho Huang David E. Ramey Shang-Tian Yang |
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Affiliation: | (1) Department of Chemical Engineering, Ohio State University, 140 West 19th Avenue, 43210 Columbus, OH;(2) Environmental Energy Inc., 253 N. Waggoner Road, PO Box 15, 43004 Blacklick, OH |
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Abstract: | We explored the influence of dilution rate and pH in continuous cultures of Clostridium acetobutylicum. A 200-mL fibrous bed bioreactor was used to produce high cell density and butyrate concentrations at pH 5.4 and 35°C. By feeding glucose and butyrate as a cosubstrate, the fermentation was maintained in the solventogenesis phase, and the optimal butanol productivity of 4.6g/(L h) and a yield of 0.42 g/g were obtained at a dilution rate of 0.9h−1 and pH 4.3. Compared to the conventional acetone-butanol-ethanol fermentation, the new fermentation process greatly improved butanol yield, making butanol production from corn an attractive alternative to ethanol fermentation. |
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Keywords: | ABE fermentation butanol Clostridium acetobutylicum fibrous bed bioreactor dilution rate |
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