Identification and determination of aminopeptidase activities secreted by lemon balm |
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| Authors: | M Korenova J Stano K Micieta V Blanarikova |
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| Institution: | (1) Garden of Medicinal Plants, Faculty of Pharmacy, Comenius University, Odbojarov 10, 832 32 Bratislava 3, Slovak Republic;(2) Department of Botany, Faculty of Natural Sciences, Comenius University, Revova 39, 811 01 Bratislava 1, Slovak Republic;(3) Department of Cell and Molecular Biology of Drugs, Comenius University, Odbojarov 10, 832 32 Bratislava 3, Slovak Republic |
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| Abstract: | Using synthetic substrates, an uncomplicated and sensitive procedure for the identification and determination of extracellular
aminopeptidase was developed. The β-naphthylamides of the amino acids were applied for the identification of extracellular
aminopeptidase, whereas the 4-(phenylazo) phenylamides of the amino acids were used for the determination of intra-and extracellular
aminopeptidase activity. The results show a 81.8–88.9% intracellular and 11.1–18.2% extracellular distribution of the studied
enzyme activity.
Published in Khimiya Prirodnykh Soedinenii, No. 2, pp. 167–169, March–April, 2007. |
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| Keywords: | identification determination aminopeptidase lemon balm |
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