固相萃取-表面增强拉曼光谱法测定奶粉中三聚氰胺

建立了固相萃取-表面增强拉曼光谱法(SPE-SERS)测定奶粉中三聚氰胺的分析方法。待测物经乙腈提取、活性碳吸附及氢氧化钠溶液洗脱后进行SERS检测。该方法的线性范围为0.005 0~1.6 mg/L,检出限为0.100 mg/kg,回收率为75.3%~125%,相对标准偏差(RSD,n=5)不大于9.3%。该法灵敏度高、杂质干扰小、结果准确可靠,能满足奶粉中三聚氰胺的快速检测,在奶粉质量监控方面具有良好的应用潜力。

Determination of Melamine in Milk Powder by Solid-phase Extraction Coupled with Surface-enhanced Raman Spectroscopy

A new analytical method was developed for the determination of melamine in milk powder by solid-phase extraction coupled with surface-enhanced Raman spectroscopy.The melamine in sample was extracted with acetonitrile,then adsorbed with activated carbon and eluted with sodium hydroxide solution.The results showed that the linear range for melamine was in the range of 0.005 0-1.6 mg/L,with a limit of detection of 0.100 mg/kg.While the method was applied in the analysis of melamine in milk powder,the recoveries for melamine were in the range of 75.3%-125%,and the relative standard deviations(n=5) were not more than 9.3%.With a remarkable effect in removing impurities,this method is accurate,reliable and sensitive in the rapid determination of melamine in milk powder,showing a great potential in monitoring the quality of milk powder.