DNA biosensors for the detection of aflatoxin producing Aspergillus flavus and A. parasiticus |
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| Authors: | Sara Tombelli Marco Mascini Barbara Scherm Gianni Battacone Quirico Migheli |
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| Affiliation: | (1) Dipartimento di Chimica, Universit? degli Studi di Firenze, Via della Lastruccia 3, Sesto Fiorentino, Italy;(2) Istituto Nazionale di Biostrutture e Biosistemi (INBB), Viale delle Medaglie d’Oro 305, Rome, Italy;(3) Dip. Protezione delle Piante/Sez. Patologia Vegetale, Facolt? di Agraria, Universit? degli Studi di Sassari, Via E. De Nicola 1, Sassari, Italy;(4) Dipartimento di Scienze Zootecniche, Facolt? di Agraria, Universit? degli Studi di Sassari, Via E. De Nicola 9, Sassari, Italy; |
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| Abstract: | Abstract In this work, we report on the development of a DNA-based piezoelectric biosensor specific for the detection of an amplicon of the aflD gene of Aspergillus flavus and A. parasiticus. Expression of this gene is consistently correlated with a strain’s ability to produce aflatoxins that are considered very potent liver carcinogens in various animal species and humans. The DNA biosensor has been characterized with synthetic oligonucleotides and amplicons. Moreover, it has been applied to the analysis of real samples consisting of amplicons of DNA extracted from flours and feed contaminated with A. flavus and A. parasiticus. |
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