Xylanase recovery effect of extraction conditions on the aqueous two-phase system using experimental design |
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Authors: | Costa Silgia A Pessoa Adalberto Roberto Inês C |
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Institution: | (1) Department of Biotechnology/FAENQUIL, Rod. Itajubá-Lorena, Km 74, 5., 12.600-000 Lorena/SP, Brazil |
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Abstract: | The partitioning of xylanase produced byPenicillium janthinellum in aqueous two-phase systems (ATPS) using poly(ethylene glycol) (PEG) and phosphate (K2HPO4/KH2PO4) was studied employing a statistical experimental design. The aim was to identify the key factors governing xylanase partitioning.
The interactions of five factors (PEG concentration molecular weight, concentration of buffer K2HPO4/KH2PO4, pH, and NaCl concentration) and their main effects on the partition coefficient (K) were evaluated by means of a 25 full-factorial experimental design with four center points. The %PEG, %NaCl, and pH were the most important factors affecting
the response variable (K). Response surface methodology (RSM) was adopted and an empirical second-order polynomial model was
constructed on the basis of the results. The optimum partition conditions were pH 7.0, PEG = 8.83% and NaCl = 6.02%. Adequacy
of the model for predicting optimum response value was tested under these conditions. The experimental xylanase partition
coefficient (K) was 2.21, whereas its value predicted by the model was 2.33. These results indicate that the predicted model
was adequate for the process. PEG molecular weight and phosphate concentration did not affect the xylanase partition coefficient. |
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Keywords: | Index Entries" target="_blank">Index Entries Aqueous two-phase system xylanase Penicillium janthinellum experimental design |
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