A METHOD FOR MEASURING PHYTOCHROME IN PLANTS GROWN IN WHITE LIGHT |
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| Authors: | Merten Jabben Gerald F. Deitzer |
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| Affiliation: | Smithsonian Institution, Radiation Biology Labora tory, 12441 Parklawn Drive, Rockville, MD 20852, U.S.A. |
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| Abstract: | Abstract— Quantitative spectrophotometric measurement of phytochrome in plants grown for relatively long periods of time in white light is not possible due to the presence of chlorophyll. A method is described that prevents the accumulation of chlorophyll permitting such direct spectrophotometric measurement in light-grown tissue. Oat seedlings grown in the presence of the herbicide San 9789† (Norflurazon) for 6 days in constant light have considerably less chlorophyll than etiolated seedlings exposed to 1 min of light. Phytochrome concentrations measured in vivo and in vitro in these herbicide-treated plants were found to be about 2% of the level in etiolated tissue. |
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