Low-magnesium, <Emphasis Type="Italic">trans</Emphasis>-cleavage activity by type III,tertiary stabilized hammerhead ribozymes with stem 1 discontinuities |
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Authors: | Email author" target="_blank">Donald?H?BurkeEmail author S?Travis?Greathouse |
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Institution: | (1) Department of Chemistry, Indiana University, Bloomington, IN, 47405-7102, U.S.A;(2) Department of Molecular Microbiology and Immunology,471h Life Sciences Center, University of Missouri-Columbia, School of Medicine, 1201 Rollins Dr., Columbia, MO, 65212-7310, U.S.A |
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Abstract: | Background Low concentrations of free magnesium in the intracellular environment can present critical limitations for hammerhead ribozymes,
especially for those that are designed for intermolecular (trans) cleavage of a host or pathogen RNA. Tertiary stabilizing motifs (TSM's) from natural and artificial ribozymes with a "type
I" topology have been exploited to stabilize trans-cleaving hammerheads. Ribozymes with "type II" or "type III" topologies might seem incompatible with conversion to trans-cleavage designs, because opening the loop at the end of stem 1 or stem 2 to accommodate substrate binding is expected to
disrupt the TSM and eliminate tertiary stabilization. |
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Keywords: | |
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