| 产腈水合酶菌Nocardia sp.HD9611超高诱导及发酵工艺改进 |
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| 引用本文： | 马武生,马同森,杨生玉.产腈水合酶菌Nocardia sp.HD9611超高诱导及发酵工艺改进[J].河南大学学报(自然科学版),2004,34(2):37-41. |
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| 作者姓名： | 马武生 马同森 杨生玉 |
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| 作者单位： | 河南大学,化学化工学院,河南,开封,475001;河南大学,生命科学学院,河南,开封,475001 |
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| 基金项目： | 河南省科技攻关项目(0424270136) |
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| 摘 要： | 研究了金属离子和诱导因子对Nocardia sp．HD9611产腈水合酶的促进作用．结果表明，培养基中加入Co^2 和脲将会对腈水合酶的诱导激活起关键作用，并且发现诱导剂A和B对腈水合酶的产生具有较强的诱导作用，当它们的质量分数分别为0．8％和0．2％时，酶活分别提高了49．4％和104．3％．新的发酵工艺采用诱导剂分批加入方式，不仅可以有效解除诱导剂对细胞生长抑制，并且酶活也有较大的提高。
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| 关 键 词： | 腈水合酶 超高诱导 丙烯酰胺 |
| 文章编号： | 1003-4978(2004)02-0037-04 |
| 修稿时间： | 2003年12月30 |
Nitrile Hydratase Super-induction and Fermentation Method Modification of Nocardia sp.HD9611 |
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| MA Wu-sheng,MA Tong-sen.Nitrile Hydratase Super-induction and Fermentation Method Modification of Nocardia sp.HD9611[J].Journal of Henan University(Natural Science),2004,34(2):37-41. |
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| Authors: | MA Wu-sheng MA Tong-sen |
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| Institution: | MA Wu-sheng~1,MA Tong-sen~ |
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| Abstract: | The effects of some metal ions and inducing factors on production of nitrile hydratase in Nocardia sp.HD9611 were studied. The results show that it is necessary to add Co~(2+ )and urea into the culture for inducing the production of nitrile hydratase. And inducer A and B have power activation for expression of nitrile hydratase. The enzyme activities have individual increasing of 0.8% and 0.2% when the mass present of inducer A was 0.8% and B was 0.2%.The new stage addition mode of inducer can not only effectively relieve inhibition but also improve the enzyme activity when fermentation method is modified. |
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| Keywords: | nitrile hydratase super-inducion acrylamide |
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