IMPAIRED REPAIR OF UVC-INDUCED DNA DAMAGE IN L5178Y-R CELLS: DNA UNWINDING STUDIES WITH THE USE OF 1-β-D-ARABINOFURANOSYL CYTOSINE |
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| Authors: | rena Szumiel Danuta Wlodek KarlJ Johanson |
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| Institution: | Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology,PL–03–185Warsaw, Poland;department of Radioecology, The Swedish University of Agricultural Sciences,S–750 07 Uppsala, Sweden |
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| Abstract: | Abstract— L5178Y-R (LY-R) and L5178Y-S (LY-S) cells differ in sensitivity to UVC radiation (D0: 2.8 and 9.0 J/m2respectively, for cells exposed in Fischer's medium). We used these cells and a DNA unwinding technique in conjunction with 1-β-D-arabinosyl cytosine to determine DNA strand breaks accumulating as a result of enzymatic incision during DNA repair. Following UVC exposure DNA strand break accumulation was observed in LY-S cells but not in LY-R cells. The repair defect in LY-R cells is accompanied by a delayed recovery of 3H]thymidine incorporation. |
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