Extension of the application of the thermal in-situ reaction on NH2 layers to the detection of catecholamines in biological materials |
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| Authors: | R Klaus W Fischer H E Hauck |
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| Institution: | (1) Fichtestraße 25, 64285 Darmstadt, Germany;(2) E. Merck, 64271 Darmstadt, Germany |
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| Abstract: | Summary This article describes a thin-layer chromatographic method for the determination of catecholamines in human urine. Chromatographic separation on NH2-modified silica gel layers is followed by in-situ visualization brought about by heating the plate. The use of this derivatization technique for other urine components, such as creatine, creatinine, uric acid and glucose, is also discussed. |
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| Keywords: | Thin-layer chromatography NH2-silica gel Catecholamines Urine samples |
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