化学交联葡萄糖基取代聚膦腈水凝胶制备和胰岛素释放研究

在合成了葡萄糖基和甲氧乙氧基共取代聚膦腈的基础上,进一步通过向聚膦腈主链引入一定量带自由氨基的取代基,与戊二醛反应交联后,获得了具有化学交联点的聚膦腈水凝胶.该水凝胶与伴刀豆球蛋白(Con A)结合后,水凝胶的溶涨平衡性能表现出对葡萄糖浓度的依赖性.将负载有胰岛素的聚膦腈水凝胶交替置于含不同葡萄糖浓度(4 mg/mL和1 mg/mL)的介质中,可检测到在高浓度葡萄糖环境下,胰岛素的释放明显加快,而当葡萄糖的浓度降低后,在一段时间内几乎检测不到胰岛素的释放,但随着浸泡时间的延长,仍会逐渐出现胰岛素从凝胶缓慢扩散释放的现象.以上研究表明,化学交联的葡萄糖基取代聚膦腈水凝胶可用于对胰岛素的葡萄糖响应释放.

PREPARATION OF A GLUCOSE-SENSITIVE POLYPHOSPHAZENE HYDROGEL AND ITS INSULIN RELEASE BEHAVIOR

A novel kind of biodegradable glucose-sensitive polyphosphazene hydrogel has been prepared basing on both the chemical crosslinking and the specific interaction between polymer-bound glucose and concanavalin A(Con A).Glucosyl-co-methoxyethoxyl-co-2-((2-aminoethoxy))ethoxyl substituted polyorgano-phosphazene was synthesized by subsequently nucleophilic substitution of polydichlorophosphazene with 2-(2-((tert-butoxycarbony1)amino)ethoxy])ethanol,diisopropylidene D-glucose and methoxyethanol,which was followe...