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压电免疫传感器用于乙肝表面抗原的测定
引用本文:陈泽忠,王柯敏,陈永康,肖丹,羊小海,邵国强.压电免疫传感器用于乙肝表面抗原的测定[J].高等学校化学学报,2002,23(6):1044-1046.
作者姓名:陈泽忠  王柯敏  陈永康  肖丹  羊小海  邵国强
作者单位:1. 化学生物传感与计量学国家重点实验室, 湖南大学化学化工学院, 长沙 410082; 2. 香港浸会大学化学系, 香港
基金项目:The Research Grants Council of the Hong Kong Special Adm inistration Region,China(No.HKBU 2 0 5 4/98p),国家杰出青年基金 (批准号 :2 982 5 110 ),湖南省自然科学基金 (批准号 :0 0 GKY10 10 )资助
摘    要:乙肝表面抗原 ( HBs Ag)的检测是临床诊断乙型肝炎的一项重要指标 .目前常用酶联免疫法和放射免疫法检测 1] ,但酶本身性质不稳定且价格昂贵、操作繁琐 ;而放射免疫法存在放射性废物难处理的局限性 .压电免疫传感器具有装置简单、价格便宜、灵敏度高、实时快速和无需标记等优点 ,广泛应用于环境监测、药物分析及微生物检测等多个领域 2~ 4 ] .本文应用自组装单分子膜技术 ,在压电石英晶体表面形成致密有序的半胱胺单分子膜 ,通过戊二醛共价交联 ,将乙肝表面抗原单克隆抗体分子固定于晶体电极表面 ,研制成 HBs Ag压电免疫传感器 ,用于…

关 键 词:压电免疫传感器  乙肝表面抗原  自组装膜  
文章编号:0251-0790(2002)06-1044-03
收稿时间:2001-03-06

Development of a Piezoelectric Immunosensor for Hepatitis B Surface Antigen
CHEN Ze Zhong ,WANG Ke Min ,CHEN Yong Kang XIAO Dan ,YANG Xiao Hai ,SHAO Guo Qiang.Development of a Piezoelectric Immunosensor for Hepatitis B Surface Antigen[J].Chemical Research In Chinese Universities,2002,23(6):1044-1046.
Authors:CHEN Ze Zhong  WANG Ke Min  CHEN Yong Kang XIAO Dan  YANG Xiao Hai  SHAO Guo Qiang
Institution:1. State Key Lab of Chem biological Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082; 2. Department of Chemistry, B aptist University, Hong Kong, China
Abstract:Apiezoelectric immunosensor has been developed for the detection of Hepatitis Bsurface antigen(HBsAg). Treatment of a Au electrode with cystamine solution results in cysteamine self-assemble monolayers(SAMs). Using a bifunctional cross-linker, glutaraldehyde, the antibody was immobilized on the electrode surface. The influence factors such as the reaction time of immunoreaction and the concentration of antibody etc. were investigated. Under the optimized experimental conditions, a series standard concentration of HBsAg solutions were analyzed in solution. The frequency shift was linear with the antigen mass concentration in the range of 0.2-1.20 μg/mL. The analysis results of two serum specimens showed that the positive serum and the negative serum could be distinguished easily. Glycine HCl solution(0.2 mol/L) was used for desorption of the bound antigen. The crystal could be regenerated nearly 8 times without detectable loss of activity.
Keywords:Piezoelectric immunosensor  Hepatieis B surface antigen  Self  assemble monolayers
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