| Abstract: | A method is described for quantitative determination of phospholipids of mitochondria following one-dimensional thin-layer chromatography without previous elution. Using high performance thin-layer chromatography (HPTLC) and an in situ fluorescence technique, the time needed for quantitative determination is relatively short. As the method is rather sensitive, small amounts of the extracts can be applied (about 200 ng of total phospholipids per spot). The reproducibility for the phospholipid fractions determined was in the range of 8–17%. The procedure was tested with lipid extracts of rat liver mitochondria. The method allows the determination of cardiolipin, phosphatidyl ethanolamine, phosphatidyl inositol, phosphatidyl choline, and sphingomyelin. |
