Residue screening for the beta-agonists clenbuterol, salbutamol and cimaterol in urine using enzyme immunoassay and high-performance liquid chromatography. |
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Authors: | H H Meyer L Rinke I Dürsch |
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Affiliation: | Institut für Physiologie, Süddeutschen Versuchs- und Forschungsanstalt für Milchwirtschaft, Technische Universit?t München, Freising-Weihenstephan, Germany. |
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Abstract: | The antibody for enzyme immunoassay was raised against clenbuterol-diazo-BSA, and salbutamol-carboxymethyl ether-biocytin was used as a label. Procedural blanks from 500 negative urine samples were always less than 0.2 ppb salbutamol or less than 0.02 ppb clenbuterol equivalents, and a residue level of 1 ppb was detected with good reliability. After treatment of veal calves with anabolic dosages, residue levels in urine amounted to 10-200 ppb clenbuterol or salbutamol. beta-Agonists were separated by high-performance liquid chromatography on LiChrospher RP-Select B columns, and acidic methanol-buffer or acetonitrile-buffer mobile phases. Combinations of high-performance liquid chromatography and enzyme immunoassay were used for confirmation. |
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