超高灵敏度荧光显微镜及其在生物学中的应用

介绍了在原有数字化高灵敏度荧光显微镜的基础上研制的超高灵敏度荧光显微镜，对荧光实现１０＾６数量级的增益，可获得细胞的光子灵敏显微图像，并结合图像融合技术，实现极微弱荧光图像的定位显示，采用具有视频速率的图像采集系统，可用于研究某区域荧光强度随时间变化的动力学过程。将该显微镜应用在生物医学领域的细胞凋亡的研究，观察到Ａｓ２Ｏ３诱导ＭＧＣ－８０３细胞调亡中ＣａＴ＾２＋浓度升高，预示着Ｃａ６２＋可能是由

Ultra-High Sensitivity Fluorescence Microscope and Its Application in Biology

Based on the digital high sensitivity fluorescence microscope, the ultra high sensitivity fluorescence microscope is developed. The system can enhance the fluorescence intensity more than 10 6 and get the photon sensitive image of ultra weak fluorescence. The accurate position of ultra weak fluorescence can be confirmed by image fusion methods. Because the speed of image acquired system is 25 frame/s, the dynamic characters of fluorescence intensity can be investigated. Using the novel microscope to study the apoptosis of MGC 803 cells induced by As 2O 3, the density of Ca 2 in apoptotic cells obviously increased. Ca 2 is probably one of important roles in the process of MGC 803 apoptosis induced by As 2O 3.