Initial Proteome Analysis of Caffeine-Induced Proteins in <Emphasis Type="Italic">Aspergillus tamarii</Emphasis> Using Two-Dimensional Fluorescence Difference Gel Electrophoresis |
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Authors: | Gerardo Gutiérrez-Sánchez James Atwood V S Kumar Kolli Sévastianos Roussos Christopher Augur |
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Institution: | 1.Institut de Recherche pour le Développement, Faculté des Sciences de Saint Jér?me; Case 441,Université Paul Cézanne,Marseille,France;2.Complex Carbohydrate Research Center,The University of Georgia,Athens,USA;3.Windber Research Institute,Windber,USA |
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Abstract: | Caffeine is toxic to most microorganisms. However, some filamentous fungi, such as Aspergillus tamarii, are able to metabolize this alkaloid when fed caffeine as the sole nitrogen source. The aim of the present work was to identify
intracellular A. tamarii proteins, regulated by caffeine, using fluorescence difference two-dimensional gel electrophoresis. Specific proteins from
two culture media of A. tamarii grown either on ammonium sulfate or caffeine as the sole nitrogen source were analysed by mass spectrometry. Thirteen out
of a total of 85 differentially expressed spots were identified after database search. Identified up-regulated proteins include
phosphoglycerate kinase, malate dehydrogenase, dyp-type peroxidase family protein, heat shock protein, Cu, Zn superoxidase
dismutase and xanthine dehydrogenase. Some of the proteins identified in this study are involved in the caffeine degradation
pathway as well as in stress response, suggesting that stress proteins could be involved in caffeine metabolism in filamentous
fungi. |
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Keywords: | |
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