基于迈克尔加成反应的沙门氏菌纳米荧光标记研究

本文通过迈克尔加成反应的荧光标记法,成功构建了纳米荧光探针标记的沙门氏菌(YB1-INPs)。采用三(2-羧乙基)膦将沙门氏菌YB1外膜蛋白表面上的二硫键温和还原为巯基后,与吲哚菁绿(ICG)@纳米探针(INPs)的马来酰亚胺基团(Mal)交联形成YB1-INPs。通过扫描电镜和共聚焦荧光成像对YB1-INPs的形貌、光学性质进行表征。通过活死细菌染色和LB琼脂平板实验对YB1-INPs的活性进行考察。结果表明,INPs成功标记到沙门氏菌YB1表面后对YB1的形态、光学性质、活性及生长均没有产生影响,并且能够用于沙门氏菌YB1体内靶向的荧光分布成像。本研究提供的这种简单、安全、高效的荧光标记方法能够将纳米材料标记到生物载体上,可以应用于生物载体的药物输送和体内监测。

Nano-Fluorescence Labeling of Salmonella Based on Michael Addition Reaction

In this paper, nano-fluorescent probes labeled salmonella YB1 - INPs was prepared by Michael addition reaction of fluorescent labeling method, which phospholipid-polymer nanoprobe (INPs) loaded with indocinine green (ICG), a near infrared fluorescent dye, were labeled to the surface of salmonella YB1which rich in free sulfhydryl group (-SH). By scanning electron microscopy (SEM) and confocal fluorescence imaging of YB1 - INPs characterizing the morphology,SopticalSpropertySand activity. The results show that nanoprobe INPs successful labeling to salmonella YB1 surface, and the mild chemical labeling had no adverse effect on the morphology, vitality and growth of YB1. The simple, safe and efficient Nano-fluorescence labeling provided in this study is a promising technique for label nanomaterials onto biological carriers, and can also be used for local drug delivery, rapid assembly and in vivo monitoring based on biological carriers.