首页 | 本学科首页   官方微博 | 高级检索  
     

高效液相色谱法同时测定秋葵干蔬中的脂溶性维生素A、E、K3
引用本文:董爱军,崔艳华,杨鑫. 高效液相色谱法同时测定秋葵干蔬中的脂溶性维生素A、E、K3[J]. 分析测试学报, 2011, 30(8): 887-891. DOI: 10.3969/j.issn.1004-4957.2011.08.011
作者姓名:董爱军  崔艳华  杨鑫
作者单位:哈尔滨工业大学食品科学与工程学院,黑龙江哈尔滨,150090
摘    要:建立了同时测定秋葵干蔬中脂溶性维生素A、E、K3的反相高效液相色谱法.样品皂化后,采用C18色谱柱(150 mm×4.6 mm,5μm i.d.)进行分离,以甲醇为流动相,流速1.0 mL/min,二极管阵列检测器(DAD)在325、290、244 nm波长下同时检测,外标法定量.结果表明,脂溶性维生素A、E、K3在0...

关 键 词:高效液相色谱  脂溶性维生素  维生素A、E、K3  秋葵

Simultaneous Determination of Fat soluble Vitamins A,E and K3 in Dried Okra by High Performance Liquid Chromatography
DONG Ai-jun,CUI Yan-hua,YANG Xin. Simultaneous Determination of Fat soluble Vitamins A,E and K3 in Dried Okra by High Performance Liquid Chromatography[J]. Journal of Instrumental Analysis, 2011, 30(8): 887-891. DOI: 10.3969/j.issn.1004-4957.2011.08.011
Authors:DONG Ai-jun  CUI Yan-hua  YANG Xin
Affiliation:DONG Ai-jun,CUI Yan-hua,YANG Xin (College of Food Science and Engineering,Harbin Institute of Technology,Harbin 150090,China)
Abstract:A method was developed for the simultaneous determination of fat-soluble vitamin A,E and K3 in dried okra by reversed phase high performance liquid chromatography coupled with diode array detection.The sample was first extracted with ethanol and followed by saponification with 50% KOH solution,then extracted with petroleum ether.The extract was separated on an Agilent Eclipse XDB C18 column(150 mm× 4.6 mm,5 μm i.d.) at 30 ℃,using methanol as mobile phase at a flow rate of 1.0 mL/min.The target compounds were simultaneously detected at 325,290,244 nm,respectively,and quantified by the external standard method.The results showed that the linear ranges for fat-soluble vitamins A,E,K3 were in the range of 0.1-20.0 mg/L with correlation coefficients more than 0.99.The spiked recoveries of three fat-soluble vitamins from dry okra sample were in the range of 88%-95% with RSDs of 0.8%-3.9%.The limits of detection(LODs,S/N=3) of VA,VE and VK3 were 0.3,0.6,0.1 mg/kg,respectively,and the limits of quantitation(LOQs,S/N=10) were 1.0,2.0,0.3 mg/kg,respectively.The developed method was simple and accurate,and could meet the demands for actual detection.
Keywords:high performance liquid chromatography(HPLC)  fat-soluble vitamins  vitamins A  E  K3  okra  
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《分析测试学报》浏览原始摘要信息
点击此处可从《分析测试学报》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号