A novel stationary phase derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed protein chromatography |
| |
Authors: | Jian-Bo Qu Xing-Zhong Wan Yan-Qin Zhai Wei-Qing Zhou Zhi-Guo Su Guang-Hui Ma |
| |
Institution: | 1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China;2. Graduate University of the Chinese Academy of Sciences, Beijing 100049, China;3. Beijing Baiao Pharmaceuticals Co., Ltd., Beijing 102200, China |
| |
Abstract: | Using agarose coated gigaporous polystyrene microspheres as a base support, a novel anion exchanger (DEAE-AP) has been developed after functionalization with diethylaminoethyl chloride. The gigaporous structure, static adsorption behavior, and chromatographic properties of DEAE-AP medium were characterized and compared with those of commercially available resin DEAE Sepharose Fast Flow (DEAE-FF). The results implied that there existed some through pores in DEAE-AP microspheres, which effectively reduced resistance to stagnant mobile phase mass transfer by inducing convective flow of mobile phase in the gigapores of medium. As a consequence, the column packed with DEAE-AP exhibited low column backpressure, high column efficiency, high dynamic binding capacity and high protein resolution at high flow velocity up to 2600 cm/h. In conclusion, all the results suggested that the gigaporous absorbent is promising for high-speed protein chromatography. |
| |
Keywords: | Gigaporous Polystyrene microspheres Anion exchanger Protein chromatography Column efficiency Dynamic binding capacity |
本文献已被 ScienceDirect 等数据库收录! |
|