Enhanced Iridium Complex Electrochemiluminescence Cytosensing and Dynamic Evaluation of Cell‐Surface Carbohydrate Expression |
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| Authors: | Dr Hong Zhou Yiying Yang Chunxiang Li Bing Yu Prof?Dr Shusheng Zhang |
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| Institution: | 1. School of Chemistry and Chemical Engineering of Linyi University, Linyi 276005 (P. R. China);2. College of Chemical and Environmental Engineering, Qingdao University, Qingdao, 266071 (P. R. China);3. Key Laboratory of Biochemistry Analysis, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042 (P. R. China) |
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| Abstract: | A newly prepared (ppy)2Ir(dcbpy)]+?PF6? (ppy: 2‐phenylpyridyl; dcbpy: 4,4′‐dicarboxy‐2,2′‐bipyridyl) and gold nanoparticle functionalized mesoporous silica nanoparticle (Au/Ir‐MSN) is reported. Based on the binding between concanavalin A (Con A) and mannose, the novel nanoparticle was applied to an ultrasensitive electrochemiluminescence (ECL) in situ cytosensing strategy and the dynamic evaluation of cell‐surface carbohydrate expression. The ECL activity of the presented Con A@Au/Ir‐MSN nanoprobe was greatly enhanced by employing a functionalized nanoparticle and graphene nanomaterial with an increased surface area and simultaneously improved electron‐transfer efficiency at the electrode interface. Under optimal conditions, the sandwich‐type ECL cytosensor showed a linear response to K562 cells at concentrations ranging from 1.0×102 to 1.0×106 cells mL?1 and realized a low detection limit of a single cell. The proposed method could also be successfully used for monitoring the dynamic variation of carbohydrate expression in cancer cells in response to external stimulation by an inhibitor. |
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| Keywords: | biosensors electrochemiluminescence gold iridium nanoparticles |
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