|
|||||
|
|
Red‐Shifted Fluorescent Aminated Derivatives of a Conformationally Locked GFP Chromophore |
|
| Authors: | Dr. Mikhail S. Baranov Dr. Kyril M. Solntsev Nadezhda S. Baleeva Dr. Alexander S. Mishin Prof. Sergey A. Lukyanov Dr. Konstantin A. Lukyanov Dr. Ilia V. Yampolsky |
| Affiliation: | 1. Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho‐Maklaya 16/10, 117997 Moscow (Russia);2. School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA 30332‐0400 (USA);3. Nizhny Novgorod State Medical Academy, 603005 Minin Sq. 10/1, Nizhny Novgorod (Russia) |
| Abstract: | A novel class of fluorescent dyes based on conformationally locked GFP chromophore is reported. These dyes are characterized by red‐shifted spectra, high fluorescence quantum yields and pH‐independence in physiological pH range. The intra‐ and intermolecular mechanisms of radiationless deactivation of ABDI‐BF2 fluorophore by selective structural locking of various conformational degrees of freedom were studied. A unique combination of solvatochromic and lipophilic properties together with “infinite” photostability (due to a dynamic exchange between free and bound dye) makes some of the novel dyes promising bioinspired tools for labeling cellular membranes, lipid drops and other organelles. |
| Keywords: | fluorescence microscopy fluorescent labeling fluorescent probes GFP chromophore solvatochromism |