Ratiometric Detection of Adenosine Triphosphate (ATP) in Water and Real‐Time Monitoring of Apyrase Activity with a Tripodal Zinc Complex |
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| Authors: | Dr Stephen J Butler |
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| Institution: | Department of Chemistry, Durham University, South Road, Durham, DH1 3LE (UK) |
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| Abstract: | Two tripodal fluorescent probes Zn?L1 , 2 have been synthesised, and their anion‐binding capabilities were examined by using fluorescence spectroscopy. Probe Zn?L1 allows the selective and ratiometric detection of adenosine triphosphate (ATP) at physiological pH, even in the presence of several competing anions, such as ADP, phosphate and bicarbonate. The probe was applied to the real‐time monitoring of the apyrase‐catalysed hydrolysis of ATP, in a medium that mimics an extracellular fluid. |
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| Keywords: | anions ATP enzymes fluorescent probes quinolines |
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