Diagnosis of corneal pathology by laser fluorescence spectroscopy |
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Authors: | V V Salmin V I Lazarenko A B Salmina M Sh Hovalyg E S Vladimirova |
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Institution: | 1.Siberian Federal University,Krasnoyarsk,Russia;2.V. F. Voino-Yasenetsky Krasnoyarsk State Medical University,Krasnoyarsk,Russia |
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Abstract: | We have studied the difference between the fluorescence spectra of the human cornea in vivo under normal conditions and after contact lenses have been worn for different lengths of time, with excitation by emission from a nitrogen laser (337 nm). The most significant sections of the difference spectrum were identified, corresponding to peaks for endogenous fluorophores (NADH and collagen). A high correlation was found between how long the contact lenses have been worn and the fluorescence intensity ratio for wavelengths 460 nm and 410 nm. |
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