| Hg2+对胡萝卜愈伤组织生长过程中POD和木质素的影响 |
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| 引用本文： | 李梦,刘同祥,孙颖,张宗申.Hg2+对胡萝卜愈伤组织生长过程中POD和木质素的影响[J].武汉大学学报(理学版),2007,53(2):239-243. |
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| 作者姓名： | 李梦 刘同祥 孙颖 张宗申 |
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| 作者单位： | 1. 大连轻工业学院,生物与食品工程学院,辽宁,大连,116034
2. 北京中医药大学,基础医学院,北京,100029 |
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| 基金项目： | 辽宁省教育厅资助项目;辽宁省大连市青年科技人才基金 |
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| 摘 要： | 经不同浓度Hg^2＋处理的胡萝卜直根，通过组织化学方法研究Hg^2＋对胡萝卜愈伤组织生长的影响．组织化学定位结果表明：同一处理样品的过氧化物酶活性随着愈伤组织的生长进程而逐渐增强，且与Hg^2＋浓度呈正相关；细胞壁中木质素沉积随着愈伤组织的生长而增加，与过氧化物酶活性变化趋势一致；Hg^2＋处理的愈伤组织中导管诱导率大于对照．结果表明过氧化物酶活性增加，进而促进木质素合成，是Hg^2＋抑制愈伤组织生长分化的原因．
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| 关 键 词： | 愈伤组织 胡萝卜 过氧化物酶 木质素 |
| 文章编号： | 1671-8836(2007)02-0239-05 |
| 修稿时间： | 2006年12月25 |
Effects of Mercury on the POD Activity and Lignin Content during the Growth of Daucus corota var. sativa Callus |
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| LI Meng,LIU Tongxiang,SUN Ying,ZHANG Zongshen.Effects of Mercury on the POD Activity and Lignin Content during the Growth of Daucus corota var. sativa Callus[J].JOurnal of Wuhan University:Natural Science Edition,2007,53(2):239-243. |
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| Authors: | LI Meng LIU Tongxiang SUN Ying ZHANG Zongshen |
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| Abstract: | Treatments with different Hg2 concentration led to reduction in the carrot callus growth,and callus growth rate correlated negatively with Hg2 concentration added.Histochemically,the results indicated that POD activity increased with the growth rate of callus within the same tissue;and lignin deposit density within the cell wall increased with the growth of callus over time,displaying a trend similar to POD activity alteration upon Hg2 imposition;Furthermore,more tracheary elements was observed in the callus tissue subjected to Hg2 than that in control.From these above data,it could be inferred that POD-promoted biosynthesis of lignin might account for the inhibition on callus differentiation by Hg2 treatment. |
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| Keywords: | Hg2 |
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