Determination of trifluorothymidine in the eye using high-performance liquid chromatography. |
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| Authors: | M R Riegel P P Ellis |
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| Institution: | Department of Ophthalmology, University of Colorado, School of Medicine, Denver 80262. |
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| Abstract: | A high-performance liquid chromatographic assay for trifluorothymidine in biological matrices of the eye is presented. Sample pretreatment was based on liquid-liquid extraction with ethyl acetate. Following evaporation, the residue was analyzed using a reversed-phase octadecylsilane column with ultraviolet detection. The lower limit of detection is ca. 50 ng/ml (ca. 3 ng on column). The method is simple, reproducible and selective. Concentrations of trifluorothymidine in the aqueous humour, vitreous humour and cornea of normal rabbits after a 50-microliters topical dose of 1% trifluorothymidine are reported. |
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