| X射线辐照引起的线粒体D310片段突变的快速检测 |
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| 引用本文： | 张昕,周鑫,张红. X射线辐照引起的线粒体D310片段突变的快速检测[J]. 原子核物理评论, 2011, 28(1): 118-121. DOI: 10.11804/NuclPhysRev.28.01.118 |
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| 作者姓名： | 张昕 周鑫 张红 |
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| 作者单位： | 1中国科学院近代物理研究所, 甘肃 兰州730000; 2中国科学院重离子束辐射生物医学重点实验室, 甘肃 兰州730000; 3甘肃省重离子束辐射医学应用基础重点实验室, 甘肃 兰州730000; 4中国科学院研究生院, 北京100049 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)项目，国家自然科学基金重点项目，甘肃省重大科技专项项目 |
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| 摘 要： | 采用4 Gy X射线辐照人乳腺癌细胞MCF 7， 分别在照射后0， 2， 4， 8， 16， 24， 48， 72和144 h时间点收集细胞， 提取DNA并以聚合酶链式反应进行扩增， 再以BsaXI限制性内切酶对扩增产物进行消化， 消化产物以琼脂糖凝胶电泳进行分离。 结果表明， 4 Gy X射线辐照可引起D310片段突变， 且该突变在经辐照之后144 h检测水平明显超过野生型。 Human breast cancer cell line MCF 7 was irradiated with 4 Gy X ray， collected at 0， 2， 4， 8， 16， 24， 48， 72, 144 h after irradiation， respectively. Whole genome DNA including mtDNA were extracted at each time point， and amplified by polymerase chain reaction(PCR). Then the PCR product was subjected to BSAXI digestion， all of digestion product then underwent a brief electrophoresis. Results showed D310 mutation can be induced by 4 Gy X ray irradiation and D310 mutation can overwhelm the normal phenotype 144 h after irradiation.
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| 关 键 词： | X射线辐照 线粒体DNA损伤 D310片段突变 聚合酶链式反应 |
| 收稿时间： | 1900-01-01 |
A Novel Approach for Rapid Detection of X-ray Irradiation Induced mtDNA D310 Mutation |
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| ZHANG Xin,ZHOU Xin,ZHANG Hong. A Novel Approach for Rapid Detection of X-ray Irradiation Induced mtDNA D310 Mutation[J]. Nuclear Physics Review, 2011, 28(1): 118-121. DOI: 10.11804/NuclPhysRev.28.01.118 |
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| Authors: | ZHANG Xin ZHOU Xin ZHANG Hong |
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| Affiliation: | 1 Institue of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;2 Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China;3 Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, China;4 Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | Human breast cancer cell line MCF 7 was irradiated with 4 Gy X ray， collected at 0， 2， 4， 8， 16， 24， 48， 72, 144 h after irradiation， respectively. Whole genome DNA including mtDNA were extracted at each time point， and amplified by polymerase chain reaction(PCR). Then the PCR product was subjected to BSAXI digestion， all of digestion product then underwent a brief electrophoresis. Results showed D310 mutation can be induced by 4 Gy X ray irradiation and D310 mutation can overwhelm the normal phenotype 144 h after irradiation. |
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