| 人参SQS基因的干扰载体构建及转化人参愈伤组织 |
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| 引用本文： | 蒋世翠,张明哲,王义,孙春玉,李校堃,张美萍.人参SQS基因的干扰载体构建及转化人参愈伤组织[J].吉林大学学报(理学版),2011,49(6):1136-1140. |
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| 作者姓名： | 蒋世翠 张明哲 王义 孙春玉 李校堃 张美萍 |
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| 作者单位： | 1. 吉林农业大学 生命科学学院, 长春 130118;2. 吉林农业大学 生物反应器与药物开发教育部工程中心, 长春 130118 |
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| 基金项目： | 吉林省科技发展计划项目(批准号:20100712;20080715); 长春市科技计划项目(批准号:长科合字2008138号;长科合字2007135号) |
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| 摘 要： | 以5年生人参为材料, 根据人参SQS基因设计正义及反义片段引物, 构建了SQS基因干扰表达载体； 利用农杆菌转化法转化人参愈伤组织, 对获得的抗性愈伤组织进行PCR检测及实时定量PCR检测, 并对抗性愈伤组织的皂苷进行HPLC分析. 实验结果表明, 与非转化人参愈伤组织相比, 转化后的愈伤组织SQS基因表达量降低, 皂苷含量有所变化. SQS是人参皂苷生物合成途径的关键酶, 抑制SQS基因的表达, 可调控人参皂苷含量变化.
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| 关 键 词： | 人参 干扰载体 SQS基因 人参愈伤组织 |
| 收稿时间： | 2010-12-29 |
Interference Vector Construction of Panax ginseng's SQS Gene and Transformation Callus |
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| JIANG Shi-cui,ZHANG Ming-zhe,WANG Yi,SUN Chun-yu,LI Xiao-kun,ZHANG Mei-ping.Interference Vector Construction of Panax ginseng's SQS Gene and Transformation Callus[J].Journal of Jilin University: Sci Ed,2011,49(6):1136-1140. |
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| Authors: | JIANG Shi-cui ZHANG Ming-zhe WANG Yi SUN Chun-yu LI Xiao-kun ZHANG Mei-ping |
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| Institution: | 1. College of Life Science, Jilin Agricultural University, Changchun 130118, China|2. Engineering Research Center ofBioreactor and Pharmaceutical Development of Ministry of Education, Jilin Agricultural University, Changchun 130118, China |
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| Abstract: | According to the SQS gene of Panax ginseng,the authors designed a sense primer and an antisense primer to construct a SQS interference expression vector.The ginseng callus was transformed by agrobacterium-mediated methods,and the selected resistant callus was assayed by PCR and real-time PCR,and the ginsenosides of resistant callus was analyzed by HPLC.Compared with the non-transformation ginseng callus,the SQS gene's expression of positive callus decreased,and the ginsenosides also had certain changes.SQS ... |
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| Keywords: | Panax ginseng interference vector SQS gene callus of Panax ginseng |
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