Comparative Analysis of the Distribution of Copper in Green and Brown Brazilian Propolis Extracts |
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| Authors: | Patrícia O Nunes Fábio I M Carvalho Joaquim A Nóbrega Kelly G Fernandes Dantas |
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| Institution: | 1.Grupo de Espectrometria Analítica Aplicada, Faculdade de Química, Instituto de Ciências Exatas e Naturais,Universidade Federal do Pará,Belém,Brazil;2.Universidade Federal Rural da Amaz?nia,Parauapebas,Brazil;3.Grupo de Análise Instrumental e Aplicada, Departamento de Química,Universidade Federal de S?o Carlos,S?o Carlos,Brazil |
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| Abstract: | We proposed here a novel analytical procedure for copper speciation in green and brown propolis extracts using SEC—HPLC—GFAAS with 0.5% m v?1 SDS in 2.5 m mol L?1 Tris–HCl (pH 7.4) as the mobile phase buffer solution. Both basic (0.05 mol L?1 NaOH) and acid (0.05 mol L?1 HCl) conditions were evaluated for sample extraction. Depending on the extraction procedure, differences in copper distribution were identified. Copper was mainly associated with high-molecular-weight (HMW) fractions in green propolis extract when extracted with basic solution, whereas with acid extraction solution, only low-molecular-weight (LMW) fractions were obtained in both samples. Furthermore, combined analysis of results obtained using SEC-UV and GF AAS confirmed the association of copper with LMW and HMW species. |
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