HTLC: A Viable Approach to Fast LC of Biogenic Amines in Food with Legacy Equipment |
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Authors: | Jian-Jun Zhong Ningbo Liao Yunfeng Pu Tian Ding Xingqian Ye Donghong Liu |
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Institution: | 1.Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R&D Center for Food Technology and Equipment,Zhejiang University,Hangzhou,China;2.Department of Nutrition and Food Safety,Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou,China;3.College of Life Sciences,Tarim University,Alar,China |
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Abstract: | Nowadays, there is a demand for fast liquid chromatography (LC) of biogenic amines in food with the equipment available in the average laboratory. To this end, high-temperature liquid chromatography (HTLC) is presented in this study as a viable option, working on a conventional LC platform at moderately high temperatures up to 80 °C. The LC platform was adapted by including an appropriate length of stainless-steel microbore tubing as a preheater. Biogenic amines as benzoyl derivatives showed good thermostability on a thermally rugged column (150?×?4.6 mm, 5 µm). As a model application the HTLC conditions were developed for wine separation. The separation selectivity changed considerably with temperature in the range 36–84 °C, and baseline resolution was obtained only at temperatures well above ambient. At 73 °C oven temperature, the system allowed a flow rate as high as 3 mL min?1 with the backpressure not exceeding 195 bar (2800 psi). The separation took less than 5 min, comparably fast to documented fast LC separations, and typically at least three to five times faster than a conventional separation. |
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