| DISCRIMINATION OF Moraxella AND Legionella pneumophila BY GAS CHROMATOGRAPHY OF WHOLE-CELL FATTY ACIDS |
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| 作者姓名： | 周方 朱厚础 唐光江 王以政 张绪团 高树德 邱明庆 钟文蓬 赵林 王陵 梁朝 |
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| 作者单位： | Institute of Microbiology and Epidemiology，Academy of Military Medical Sciences，PLA，Beijing，Institute of Microbiology and Epidemiology，Academy of Military Medical Sciences，PLA，Beijing，Institute of Microbiology and Epidemiology，Academy of Military Medical Sciences，PLA，Beijing，Institute of Microbiology and Epidemiology，Academy of Military Medical Sciences，PLA，Beijing，Institute of Microbiology and Epidemiology，Academy of Military Medical Sciences，PLA，Beijing，Institute of Microbiology and Epidemiology，Academy of Military Medical Sciences，PLA，Beijing，Moraxella and Allied Bacteria Reference Laboratory of CMCC(B). Jiangxi Institute of Medical Sciences，Nanchang，Moraxella and Allied Bacteria Reference Laboratory of CMCC(B). Jiangxi Institute of Medical Sciences，Nanchang，Moraxella and Allied Bacteria Reference Laboratory of CMCC(B). Jiangxi Institute of Medical Sciences，Nanchang，Moraxella and Allied Bacteria Reference Laboratory of CMCC(B). Jiangxi Institute of Medical Sciences，Nanchang，Moraxella and Allied Bacteria Reference Laboratory of CMCC(B). Jiangxi Institute of Medical Sciences，Nanchang |
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| 基金项目： | The project is supported by the Science Fund of the Chinese Academy of Sciences. |
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| 摘 要： | Whole-cell fatty acid profiles of 36 strains of Moraxella and its related bacteria, and 13 strainsof Legionella pneumophila were obtained by capillary gas chromatography (GC). Some component peaksin these profiles were analyzed by gas chromatography-mass spectrometry (GC-MS). Hierarchical clus-tering analysis of these bacterial fatty acid patterns was done with a microcomputer. Results showedthat these were some obvious differences between the two new species of Moraxella isolated in China,i.e. M. amylolytica and M. nanchang, and the currently established reference strains of this genus. Themajor fatty acids of these two new species were the isomers of pentadecanoic (C_(15:0)) acids. Theprofiles of L. pneumophila NJ 8331 and LDB 4_a recently isolated in China were essentially similar tothose of international reference strains type 1-8 (CDC, USA), which contained mainly 16-carbon (C_(16))acids, whereas another isolate LDB 4b was obviously dissimilar. The results of this method of discrim-ination and of t
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