Bioinspired,Size‐Tunable Self‐Assembly of Polymer–Lipid Bilayer Nanodiscs |
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Authors: | Dr Thirupathi Ravula Dr Sudheer Kumar Ramadugu Giacomo Di?Mauro Prof Ayyalusamy Ramamoorthy |
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Institution: | Biophysics Program and Department of Chemistry, The University of Michigan, Ann Arbor, MI, 48109-1055 USA |
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Abstract: | Polymer‐based nanodiscs are valuable tools in biomedical research that can offer a detergent‐free solubilization of membrane proteins maintaining their native lipid environment. Herein, we introduce a novel ca. 1.6 kDa SMA‐based polymer with styrene:maleic acid moieties that can form nanodiscs containing a planar lipid bilayer which are useful to reconstitute membrane proteins for structural and functional studies. The physicochemical properties and the mechanism of formation of polymer‐based nanodiscs are characterized by light scattering, NMR, FT‐IR, and TEM. A remarkable feature is that nanodiscs of different sizes, from nanometer to sub‐micrometer diameter, can be produced by varying the lipid‐to‐polymer ratio. The small‐size nanodiscs (up to ca. 30 nm diameter) can be used for solution NMR spectroscopy studies whereas the magnetic‐alignment of macro‐nanodiscs (diameter of > ca. 40 nm) can be exploited for solid‐state NMR studies on membrane proteins. |
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Keywords: | Lipide Membranproteine Nanoscheiben NMR-Spektroskopie Polymere |
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