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Studying solutions at high shear rates: a dedicated microfluidics setup |
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| Authors: | D. C. F. Wieland V. M. Garamus T. Zander C. Krywka M. Wang A. Dedinaite P. M. Claesson R. Willumeit-Römer |
| Affiliation: | 1. Institute for Materials Research, Helmholtz-Zentrum Geestacht: Centre for Materials and Coast Research, Max-Planck-Strasse 1, Geesthacht21502, Germany;2. Department of Chemistry, Surface and Corrosion Science, KTH Royal Institute of Technology, Drottning Kristinas v?g 51, Stockholm10044, Sweden;3. SP Chemistry, Materials and Surfaces, SP Technical Research Institute of Sweden, Box 4. 5607, StockholmSE-114 86, Sweden |
| Abstract: | The development of a dedicated small‐angle X‐ray scattering setup for the investigation of complex fluids at different controlled shear conditions is reported. The setup utilizes a microfluidics chip with a narrowing channel. As a consequence, a shear gradient is generated within the channel and the effect of shear rate on structure and interactions is mapped spatially. In a first experiment small‐angle X‐ray scattering is utilized to investigate highly concentrated protein solutions up to a shear rate of 300000 s?1. These data demonstrate that equilibrium clusters of lysozyme are destabilized at high shear rates. |
| Keywords: | shear complex solutions small‐angle X‐ray scattering (SAXS) microfluidics lysozyme aggregation rheology protein interaction beamline setup |