| 分枝杆菌Mycobacterium sp.M1甾酮C1,2位脱氢酶活性的研究 |
| |
| 引用本文： | 黄巍,别松涛,王超,路福平.分枝杆菌Mycobacterium sp.M1甾酮C1,2位脱氢酶活性的研究[J].天津科技大学学报,2005,20(4):14-17. |
| |
| 作者姓名： | 黄巍 别松涛 王超 路福平 |
| |
| 作者单位： | 天津科技大学生物工程学院,天津,300222 |
| |
| 基金项目： | 天津市自然科学基金资助项目(043606911) |
| |
| 摘 要： | 分枝杆菌Mycobacterium sp．M1可以有效降解大豆甾醇侧链得到雄甾-4-烯-3，17-二酮（AD）和雄甾-1，4-二烯-3，17-二酮（ADD）。通过该菌株细胞和细胞裂解液对底物AD的转化，表明了该菌株中存在甾酮C1．2位脱氢酶。利用酶活测定中活力高的细胞裂解液可以有效地降解大豆甾醇生成ADD。
|
| 关 键 词： | 分枝杆菌 大豆甾醇 甾酮C1 2位脱氢酶 雄甾4-烯-3 17-二N 雄甾-1 4-二烯-3 17-二酮 |
| 文章编号： | 1672-6510(2005)04-0014-04 |
| 修稿时间： | 2005年1月4日 |
Study on 3-ketosteroid-l-dehydrogenase Activity of Mycobacterium sp. M1 |
| |
| HUANG Wei,BIE Song-tao,WANG Chao,LU Fu-ping.Study on 3-ketosteroid-l-dehydrogenase Activity of Mycobacterium sp. M1[J].Journal of Tianjin University of Science & Technology,2005,20(4):14-17. |
| |
| Authors: | HUANG Wei BIE Song-tao WANG Chao LU Fu-ping |
| |
| Abstract: | The selective side-chain cleavage of stigmosterol to androsta-1-diene-3 ,17-dione( AD)and androsta-1-diene-3 ,17-dione( ADD) by Mycobacterium sp. M1 was described. Distinct 3-ketosteroid-l-dehydrogenase activities in]Mycobacterium sp. Ml were found during the transformation of AD to ADD by the cells and cell extracts. According to the assay of 3-ketosteroid-1-dehydrogenase activity, the cell extracts of Mycobacterium sp. Ml containing high 3-ketosteroid-1-dehydrogenase activity were prepared which had the ability to convert stigmosterol to ADD effectively. |
| |
| Keywords: | Mycobacterium stigmosterol 3-ketosteroid-1-dehydrogenase androsta-1 -ene-3 17-di-one androsta-1 4-diene-3 17-dione |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
