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Constant-wavelength synchronous spectrofluorimetry for determination of riboflavin in anchovies
Authors:T. J. López y López-Leytón  M. A. Lage Yusty  M. E. Alvarez Piñeiro
Affiliation:Dpto de Q. Analítica, Nutrición y Bromatología, Area Nutrición y Bromatología, Facultad de Farmacia, Universidad de Santiago de Compostela, Campus, s/n, E-15706-Santiago de Compostela, Spain, ES
Instituto de Investigación y Análisis Alimentarios, Laboratorio de Bromatología, Facultad de Farmacia, Universidad de Santiago de Compostela, Campus, s/n, E-15706-Santiago de Compostela, Spain, ES
Abstract:Constant-wavelength synchronous spectrofluorimetry was used for the analysis of riboflavin in anchovies. Synchronous fluorescence spectra were recorded between 300 and 600 nm at a scan rate of 240 nm min–1 and with excitation and emission slit-widths both set to 5 nm. The excitation-emission wavelength difference was 65 nm. The fluorescence was measured by peak-area base between 430–509 nm. Recovery was higher than 90.8%.
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