Chip-calorimetric monitoring of biofilm eradication with bacteriophages reveals an unexpected infection-related heat profile |
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Authors: | Frida Mariana Morais Friederike Buchholz Tom Hartmann Johannes Lerchner Thomas R. Neu Bärbel Kiesel Hauke Harms Thomas Maskow |
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Affiliation: | 1. Department of Environmental Microbiology, UFZ-Helmholtz Centre for Environmental Research, Leipzig, Germany 2. Institute of Physical Chemistry, TU Bergakademie Freiberg, Freiberg, Germany 3. Department of River Ecology, UFZ-Helmholtz Centre for Environmental Research, Magdeburg, Germany
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Abstract: | Bacteriophages or phage-derived biological structures are a promising alternative to the application of antibiotics to eradicate biofilms. These countermeasures are highly cell specific. For a better understanding of the sequence of the underlying processes (attachment, infection, multiplication, phage release), for optimization of phage applications, or simply for screening of suitable phages or phage-derived enzymes, real-time monitoring devices are urgently required. Calorimetry is promising because it is non-invasive and quantitatively connected to the metabolic fluxes. Chip-calorimetry provides real-time information about biofilm eradication by phages. This was confirmed by comparison with reference analyses (i.e., confocal laser scanning microscopy, colony plate counts, or phage titre determination). Furthermore, chip-calorimetry provides additional information which was not captured by the reference methods such as the enhanced cell-specific heat production caused by the infection process and a residual activity of seemingly persistent bacteria. |
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