Biotransformation of uridine monophosphate (UMP) and glucose to uridine diphosphate-glucose (UDPG) byCandida saitoana KCTC7249 cells |
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Authors: | Jeong Heon Ko Heung-Sop Shin Yu Sam Kim Dae-Sil Lee Cheorl-Ho Kim |
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Institution: | (1) Korea Research Institute of Bioscience and Biotechnology, 305-600 Taejon, Korea;(2) Department of Biochemistry, Yonsei University, Seoul, Korea;(3) Department of Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University, 780-714 Kyung-Ju City, Kungpook, Korea |
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Abstract: | The present study investigates the biotransformation of glucose with uridine monophosphate (UMP) to obtain sugar nucleotide,
UDP-glucose (UDPG), by the dried cells ofCandida saitoana KCTC7249. The biotransformation was optimized by varying the concentrations of substrates and phosphate ion. UDPG (24 mM)
was biotransformed from 200 mM glucose and 37.5 mM UMP by dried cells of C.saitoana. The glucose yields about 64% UDP-glucose, based on UMP concentration. The addition of glucose-1-phosphate to the reaction
mixture accelerated the formation of UDPG from a concentration of UMP. The structure of UDP-glucose obtained was determined
with13C NMR and FAB mass spectra. These results indicate that the yeast-dried cells could be used for the production of nucleotide
sugars for donor molecules of complex carbohydrate synthesis. |
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Keywords: | Uridine diphosphate-glucose biotransformation C saitoana |
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