Photodamaging Effects of Porphyrin in a Human Carcinoma Cell Line |
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Authors: | M Gabriela Alvarez Mariángeles La Penna E Ines Yslas Viviana Rivarola Edgardo N Durantini |
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Institution: | (1) Departamento de Biologia Molecular, Universidad Nacional de Rio Cuarto, Agenda Postal Nro 3, Rio Cuarto, 5800, Argentina;(2) Departamento de Química y Física, Universidad Nacional de Rio Cuarto, Agenda Postal Nro 3, Rio Cuarto, 5800, Argentina |
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Abstract: | A convenient procedure to visualize the photodynamic effect of porphyrins on cell lines is shown. 5,10,15,20-Tetra(4-methoxyphenyl)porphyrin (TMP) was used as photosensitizer. The culture flasks bearing a Hep-2 cell line were incubated for 24 h with 1 M of TMP. Under these conditions saturation of the TMP intracellular concentration is obtained. The irradiation of cell cultures for 30 min produces 90% cell mortality, while no toxicity was observed under dark conditions or under irradiation without TMP. This methodology can be used to demonstrate the photodynamic therapy (PDT) process in a laboratory experiment. |
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