| 瘦肉型猪(PIC344)精液酸性磷酸酶功能基团的化学修饰 |
| |
| 引用本文： | 姜骅,张勇侠,李佳,国锦琳,肖舸,刘克武. 瘦肉型猪(PIC344)精液酸性磷酸酶功能基团的化学修饰[J]. 化学研究与应用, 2005, 17(1): 36-38 |
| |
| 作者姓名： | 姜骅 张勇侠 李佳 国锦琳 肖舸 刘克武 |
| |
| 作者单位： | 四川大学生命科学院,四川,成都,610064;四川抗菌素工业研究所,四川,成都,610051 |
| |
| 基金项目： | 四川省成都市大熊猫繁殖基地基金资助项目 |
| |
| 摘 要： | 不同浓度化学修饰剂二巯基苏糖醇(DTT)、对氯汞苯甲酸(pCMB)、N-溴代琥珀酰亚胺(NBS)、苯甲基磺酰氟(PMSF)、溴代乙酸(BrAc)的修饰能抑制瘦肉型猪(PIC344)精液酸性磷酸酶(ACPase，E．C．3．1．3．2)的活力。同一浓度不同种类的修饰剂修饰酶活力降低程度不同。化学修饰剂修饰ACPase不同时间后，酶活力均有不同程度的降低，其中PMSF作用60min后酶活力仅存18％，而BrAe的修饰对ACPase的活力无明显影响。
|
| 关 键 词： | 瘦肉型猪精液(PIC344) 酸性磷酸酶 化学修饰 |
| 文章编号： | 1004-1656(2005)01-0036-03 |
| 修稿时间： | 2004-06-07 |
Effect of chemical reagents on acide phosphatase from sperm of PIC pigs(PIC344) |
| |
| JIANG Hua,ZHANG Yong-xia,LI Jia,GUO Jin-lin,XIAO Ge,LIU Ke-wu. Effect of chemical reagents on acide phosphatase from sperm of PIC pigs(PIC344)[J]. Chemical Research and Application, 2005, 17(1): 36-38 |
| |
| Authors: | JIANG Hua ZHANG Yong-xia LI Jia GUO Jin-lin XIAO Ge LIU Ke-wu |
| |
| Affiliation: | JIANG Hua~1,ZHANG Yong-xia~2,LI Jia~1,GUO Jin-lin~1,XIAO Ge~1,LIU Ke-wu~ |
| |
| Abstract: | Acid Phosphatase(ACPase,E.C.3.1.3.2) was modified by NBS,PMSF,pCMB,DTT and AcBr.The effects of differert concentration and time on the ACPase catalytic activities were determined.The reactions of ACPase from PCI344 with NBS,PMSF,pCMB and DTT resulted in a strong inhibition of enzyme activities.The activities of ACPase decreased steadily with the increase of modification reagents concentration and time.Histidine residues modified by BrAc did not change the activity of the enzyme,while the activity only remained 18%after PMSF modified ACPase for 60min. |
| |
| Keywords: | PIC pic Sperm Acid phosphatase Chemical modification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
