Direct electron transfer from modified glassy carbon electrodes carrying covalently immobilised mediators to a dissolved viologen accepting pyridine nucleotide oxidoreductase and dihydrolipoamide dehydrogenase |
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Authors: | A S Paxinos H Günther D J M Schmedding H Simon |
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Abstract: | N,N′-Diaminopropyl-4,4′-bipyridinium dication (DAPV) and cobalt diaminosarcophagine (Co—diamsar) were covalently bound on functionalised glassy carbon electrodes. The electron transfer from the modified electrodes to dissolved dihydrolipoamide dehydrogenase (Lip-DH) or viologen accepting pyridine nucleotide oxidoreductase (VAPOR) was tested by NADH formation in potentiostatically controlled electrochemical cells. The NADH formed was used to reduce pyruvate to
-lactate catalysed by
-lactate dehydrogenase (
-LDH). The reaction kinetics between the modified electrodes and the dissolved enzymes have been examined. |
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