FT-IR spectra of proteins at low temperatures |
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| Authors: | Hector L. Casal Ulrich Köhler Henry H. Mantsch Jose L. R. Arrondo |
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| Affiliation: | (1) Division of Chemistry, National Research Council of Canada, K1A OR6 Ottawa, Canada;(2) Departamento de Bioquímica, Universidad del País Vasco, E-48080 Bilbao, Spain |
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| Abstract: | Infrared spectra of several globular proteins have been recorded in fluid solutions (hydro-alcoholic mixtures) in the temperature range –110° C to 30° C. In the case of hemoglobin, which has a highly ordered secondary structure, smaller spectral changes are observed which are compatible with distortions of its helices. In contrast to this, -casein, a protein with mostly unordered structure, undergoes a much larger change whereby low temperature induces folding of the protein chains.Issued as NRCC No. 28929 |
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| Keywords: | infrared protein conformation cryodenaturation |
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